Wed 14 Jul 2021 05:54:56 PM EDT ; Analyzing system KO_NA1 Forward sequence file has been detected. Running analysis in single_end mode mkdir: created directory 'KO_NA1/QC-LOG' Wed 14 Jul 2021 05:54:56 PM EDT ; a. ---> Check read quality using FASTQC: results are stored to KO_NA1/QC-LOG Wed 14 Jul 2021 05:54:56 PM EDT ; FASTQC logs are written to KO_NA1/QC-LOG/Log.out Wed 14 Jul 2021 06:00:05 PM EDT ; Completed FASTQC Analysis mkdir: created directory 'KO_NA1/STAR' Wed 14 Jul 2021 06:00:05 PM EDT ; b. ---> Running STAR aligner Wed 14 Jul 2021 06:00:05 PM EDT ; STAR logs are written to KO_NA1/STAR/Log.out Wed 14 Jul 2021 06:23:26 PM EDT ; Completed STAR alignment Wed 14 Jul 2021 06:23:26 PM EDT ; c1. ---> Extracting primary reads from the aligned bam file Wed 14 Jul 2021 06:24:14 PM EDT ; c2. ---> Sorting primary BAM Wed 14 Jul 2021 06:24:32 PM EDT ; c3. ---> Generating Index for PCR duplicate free sorted BAM Wed 14 Jul 2021 06:24:33 PM EDT ; Completed BAM file generation mkdir: created directory 'KO_NA1/BIGWIG' Wed 14 Jul 2021 06:24:33 PM EDT ; d. ---> Convering to BAM to bigwig Wed 14 Jul 2021 06:34:56 PM EDT ; Completed conversion to bigwig mkdir: created directory 'KO_NA1/STRINGTIE' Wed 14 Jul 2021 06:34:56 PM EDT ; e. ---> Running stringtie assembler with reference annotation Wed 14 Jul 2021 06:35:24 PM EDT ; Completed stringtie assembly mkdir: created directory 'KO_NA1/CUFFLINKS' Wed 14 Jul 2021 06:35:24 PM EDT ; f. ---> Running cufflinks on KO_NA1 Completed cufflinks assembly mkdir: created directory 'KO_NA1/CUFFQUANT' Wed 14 Jul 2021 06:38:07 PM EDT ; g. ---> Running cuffquant on KO_NA1 Wed 14 Jul 2021 06:40:31 PM EDT ; Completed cuffquant mkdir: created directory 'KO_NA1/CUFFNORM' Wed 14 Jul 2021 06:40:31 PM EDT ; h. ---> Running cuffnorm on KO_NA1 Wed 14 Jul 2021 06:41:07 PM EDT ; Completed cuffnorm Wed 14 Jul 2021 06:41:07 PM EDT ; completed analysis for system : KO_NA1