Wed 14 Jul 2021 07:23:05 PM EDT ; Analyzing system KO_NA3 Forward sequence file has been detected. Running analysis in single_end mode mkdir: created directory 'KO_NA3/QC-LOG' Wed 14 Jul 2021 07:23:05 PM EDT ; a. ---> Check read quality using FASTQC: results are stored to KO_NA3/QC-LOG Wed 14 Jul 2021 07:23:05 PM EDT ; FASTQC logs are written to KO_NA3/QC-LOG/Log.out Wed 14 Jul 2021 07:28:00 PM EDT ; Completed FASTQC Analysis mkdir: created directory 'KO_NA3/STAR' Wed 14 Jul 2021 07:28:00 PM EDT ; b. ---> Running STAR aligner Wed 14 Jul 2021 07:28:00 PM EDT ; STAR logs are written to KO_NA3/STAR/Log.out Wed 14 Jul 2021 07:48:52 PM EDT ; Completed STAR alignment Wed 14 Jul 2021 07:48:52 PM EDT ; c1. ---> Extracting primary reads from the aligned bam file Wed 14 Jul 2021 07:50:00 PM EDT ; c2. ---> Sorting primary BAM Wed 14 Jul 2021 07:50:17 PM EDT ; c3. ---> Generating Index for PCR duplicate free sorted BAM Wed 14 Jul 2021 07:50:18 PM EDT ; Completed BAM file generation mkdir: created directory 'KO_NA3/BIGWIG' Wed 14 Jul 2021 07:50:18 PM EDT ; d. ---> Convering to BAM to bigwig Wed 14 Jul 2021 08:01:57 PM EDT ; Completed conversion to bigwig mkdir: created directory 'KO_NA3/STRINGTIE' Wed 14 Jul 2021 08:01:57 PM EDT ; e. ---> Running stringtie assembler with reference annotation Wed 14 Jul 2021 08:02:25 PM EDT ; Completed stringtie assembly mkdir: created directory 'KO_NA3/CUFFLINKS' Wed 14 Jul 2021 08:02:25 PM EDT ; f. ---> Running cufflinks on KO_NA3 Completed cufflinks assembly mkdir: created directory 'KO_NA3/CUFFQUANT' Wed 14 Jul 2021 08:04:59 PM EDT ; g. ---> Running cuffquant on KO_NA3 Wed 14 Jul 2021 08:07:13 PM EDT ; Completed cuffquant mkdir: created directory 'KO_NA3/CUFFNORM' Wed 14 Jul 2021 08:07:13 PM EDT ; h. ---> Running cuffnorm on KO_NA3 Wed 14 Jul 2021 08:07:49 PM EDT ; Completed cuffnorm Wed 14 Jul 2021 08:07:49 PM EDT ; completed analysis for system : KO_NA3