Wed 14 Jul 2021 08:50:16 PM EDT ; Analyzing system WT_GAQ2 Forward sequence file has been detected. Running analysis in single_end mode mkdir: created directory 'WT_GAQ2/QC-LOG' Wed 14 Jul 2021 08:50:17 PM EDT ; a. ---> Check read quality using FASTQC: results are stored to WT_GAQ2/QC-LOG Wed 14 Jul 2021 08:50:17 PM EDT ; FASTQC logs are written to WT_GAQ2/QC-LOG/Log.out Wed 14 Jul 2021 08:53:06 PM EDT ; Completed FASTQC Analysis mkdir: created directory 'WT_GAQ2/STAR' Wed 14 Jul 2021 08:53:06 PM EDT ; b. ---> Running STAR aligner Wed 14 Jul 2021 08:53:06 PM EDT ; STAR logs are written to WT_GAQ2/STAR/Log.out Wed 14 Jul 2021 09:12:24 PM EDT ; Completed STAR alignment Wed 14 Jul 2021 09:12:24 PM EDT ; c1. ---> Extracting primary reads from the aligned bam file Wed 14 Jul 2021 09:13:01 PM EDT ; c2. ---> Sorting primary BAM Wed 14 Jul 2021 09:13:14 PM EDT ; c3. ---> Generating Index for PCR duplicate free sorted BAM Wed 14 Jul 2021 09:13:15 PM EDT ; Completed BAM file generation mkdir: created directory 'WT_GAQ2/BIGWIG' Wed 14 Jul 2021 09:13:15 PM EDT ; d. ---> Convering to BAM to bigwig Wed 14 Jul 2021 09:23:19 PM EDT ; Completed conversion to bigwig mkdir: created directory 'WT_GAQ2/STRINGTIE' Wed 14 Jul 2021 09:23:19 PM EDT ; e. ---> Running stringtie assembler with reference annotation Wed 14 Jul 2021 09:23:45 PM EDT ; Completed stringtie assembly mkdir: created directory 'WT_GAQ2/CUFFLINKS' Wed 14 Jul 2021 09:23:45 PM EDT ; f. ---> Running cufflinks on WT_GAQ2 Completed cufflinks assembly mkdir: created directory 'WT_GAQ2/CUFFQUANT' Wed 14 Jul 2021 09:26:13 PM EDT ; g. ---> Running cuffquant on WT_GAQ2 Wed 14 Jul 2021 09:28:16 PM EDT ; Completed cuffquant mkdir: created directory 'WT_GAQ2/CUFFNORM' Wed 14 Jul 2021 09:28:16 PM EDT ; h. ---> Running cuffnorm on WT_GAQ2 Wed 14 Jul 2021 09:28:52 PM EDT ; Completed cuffnorm Wed 14 Jul 2021 09:28:52 PM EDT ; completed analysis for system : WT_GAQ2