Wed 14 Jul 2021 11:02:44 PM EDT ; Analyzing system WT_LPS2 Forward sequence file has been detected. Running analysis in single_end mode mkdir: created directory 'WT_LPS2/QC-LOG' Wed 14 Jul 2021 11:02:44 PM EDT ; a. ---> Check read quality using FASTQC: results are stored to WT_LPS2/QC-LOG Wed 14 Jul 2021 11:02:44 PM EDT ; FASTQC logs are written to WT_LPS2/QC-LOG/Log.out Wed 14 Jul 2021 11:06:27 PM EDT ; Completed FASTQC Analysis mkdir: created directory 'WT_LPS2/STAR' Wed 14 Jul 2021 11:06:27 PM EDT ; b. ---> Running STAR aligner Wed 14 Jul 2021 11:06:27 PM EDT ; STAR logs are written to WT_LPS2/STAR/Log.out Wed 14 Jul 2021 11:27:22 PM EDT ; Completed STAR alignment Wed 14 Jul 2021 11:27:22 PM EDT ; c1. ---> Extracting primary reads from the aligned bam file Wed 14 Jul 2021 11:28:02 PM EDT ; c2. ---> Sorting primary BAM Wed 14 Jul 2021 11:28:17 PM EDT ; c3. ---> Generating Index for PCR duplicate free sorted BAM Wed 14 Jul 2021 11:28:18 PM EDT ; Completed BAM file generation mkdir: created directory 'WT_LPS2/BIGWIG' Wed 14 Jul 2021 11:28:18 PM EDT ; d. ---> Convering to BAM to bigwig Wed 14 Jul 2021 11:40:34 PM EDT ; Completed conversion to bigwig mkdir: created directory 'WT_LPS2/STRINGTIE' Wed 14 Jul 2021 11:40:34 PM EDT ; e. ---> Running stringtie assembler with reference annotation Wed 14 Jul 2021 11:41:01 PM EDT ; Completed stringtie assembly mkdir: created directory 'WT_LPS2/CUFFLINKS' Wed 14 Jul 2021 11:41:01 PM EDT ; f. ---> Running cufflinks on WT_LPS2 Completed cufflinks assembly mkdir: created directory 'WT_LPS2/CUFFQUANT' Wed 14 Jul 2021 11:43:36 PM EDT ; g. ---> Running cuffquant on WT_LPS2 Wed 14 Jul 2021 11:45:40 PM EDT ; Completed cuffquant mkdir: created directory 'WT_LPS2/CUFFNORM' Wed 14 Jul 2021 11:45:40 PM EDT ; h. ---> Running cuffnorm on WT_LPS2 Wed 14 Jul 2021 11:46:16 PM EDT ; Completed cuffnorm Wed 14 Jul 2021 11:46:16 PM EDT ; completed analysis for system : WT_LPS2