Wed 14 Jul 2021 11:46:16 PM EDT ; Analyzing system WT_LPS3 Forward sequence file has been detected. Running analysis in single_end mode mkdir: created directory 'WT_LPS3/QC-LOG' Wed 14 Jul 2021 11:46:16 PM EDT ; a. ---> Check read quality using FASTQC: results are stored to WT_LPS3/QC-LOG Wed 14 Jul 2021 11:46:16 PM EDT ; FASTQC logs are written to WT_LPS3/QC-LOG/Log.out Wed 14 Jul 2021 11:51:18 PM EDT ; Completed FASTQC Analysis mkdir: created directory 'WT_LPS3/STAR' Wed 14 Jul 2021 11:51:18 PM EDT ; b. ---> Running STAR aligner Wed 14 Jul 2021 11:51:18 PM EDT ; STAR logs are written to WT_LPS3/STAR/Log.out Thu 15 Jul 2021 12:17:12 AM EDT ; Completed STAR alignment Thu 15 Jul 2021 12:17:12 AM EDT ; c1. ---> Extracting primary reads from the aligned bam file Thu 15 Jul 2021 12:20:51 AM EDT ; c2. ---> Sorting primary BAM Thu 15 Jul 2021 12:21:21 AM EDT ; c3. ---> Generating Index for PCR duplicate free sorted BAM Thu 15 Jul 2021 12:21:22 AM EDT ; Completed BAM file generation mkdir: created directory 'WT_LPS3/BIGWIG' Thu 15 Jul 2021 12:21:22 AM EDT ; d. ---> Convering to BAM to bigwig Thu 15 Jul 2021 12:30:38 AM EDT ; Completed conversion to bigwig mkdir: created directory 'WT_LPS3/STRINGTIE' Thu 15 Jul 2021 12:30:38 AM EDT ; e. ---> Running stringtie assembler with reference annotation Thu 15 Jul 2021 12:31:08 AM EDT ; Completed stringtie assembly mkdir: created directory 'WT_LPS3/CUFFLINKS' Thu 15 Jul 2021 12:31:08 AM EDT ; f. ---> Running cufflinks on WT_LPS3 Completed cufflinks assembly mkdir: created directory 'WT_LPS3/CUFFQUANT' Thu 15 Jul 2021 12:33:52 AM EDT ; g. ---> Running cuffquant on WT_LPS3 Thu 15 Jul 2021 12:36:19 AM EDT ; Completed cuffquant mkdir: created directory 'WT_LPS3/CUFFNORM' Thu 15 Jul 2021 12:36:19 AM EDT ; h. ---> Running cuffnorm on WT_LPS3 Thu 15 Jul 2021 12:36:55 AM EDT ; Completed cuffnorm Thu 15 Jul 2021 12:36:55 AM EDT ; completed analysis for system : WT_LPS3