Thu 15 Jul 2021 01:10:29 AM EDT ; Analyzing system WT_NA2 Forward sequence file has been detected. Running analysis in single_end mode mkdir: created directory 'WT_NA2/QC-LOG' Thu 15 Jul 2021 01:10:29 AM EDT ; a. ---> Check read quality using FASTQC: results are stored to WT_NA2/QC-LOG Thu 15 Jul 2021 01:10:29 AM EDT ; FASTQC logs are written to WT_NA2/QC-LOG/Log.out Thu 15 Jul 2021 01:14:00 AM EDT ; Completed FASTQC Analysis mkdir: created directory 'WT_NA2/STAR' Thu 15 Jul 2021 01:14:00 AM EDT ; b. ---> Running STAR aligner Thu 15 Jul 2021 01:14:00 AM EDT ; STAR logs are written to WT_NA2/STAR/Log.out Thu 15 Jul 2021 01:38:24 AM EDT ; Completed STAR alignment Thu 15 Jul 2021 01:38:24 AM EDT ; c1. ---> Extracting primary reads from the aligned bam file Thu 15 Jul 2021 01:39:16 AM EDT ; c2. ---> Sorting primary BAM Thu 15 Jul 2021 01:39:35 AM EDT ; c3. ---> Generating Index for PCR duplicate free sorted BAM Thu 15 Jul 2021 01:39:36 AM EDT ; Completed BAM file generation mkdir: created directory 'WT_NA2/BIGWIG' Thu 15 Jul 2021 01:39:36 AM EDT ; d. ---> Convering to BAM to bigwig Thu 15 Jul 2021 01:50:03 AM EDT ; Completed conversion to bigwig mkdir: created directory 'WT_NA2/STRINGTIE' Thu 15 Jul 2021 01:50:03 AM EDT ; e. ---> Running stringtie assembler with reference annotation Thu 15 Jul 2021 01:50:33 AM EDT ; Completed stringtie assembly mkdir: created directory 'WT_NA2/CUFFLINKS' Thu 15 Jul 2021 01:50:33 AM EDT ; f. ---> Running cufflinks on WT_NA2 Completed cufflinks assembly mkdir: created directory 'WT_NA2/CUFFQUANT' Thu 15 Jul 2021 01:53:19 AM EDT ; g. ---> Running cuffquant on WT_NA2 Thu 15 Jul 2021 01:55:48 AM EDT ; Completed cuffquant mkdir: created directory 'WT_NA2/CUFFNORM' Thu 15 Jul 2021 01:55:48 AM EDT ; h. ---> Running cuffnorm on WT_NA2 Thu 15 Jul 2021 01:56:23 AM EDT ; Completed cuffnorm Thu 15 Jul 2021 01:56:23 AM EDT ; completed analysis for system : WT_NA2