Host version information: Castle.Core: 3.3.0.0 Castle.Windsor: 3.3.0.0 Illumina.BaseSpace.NativeApp.Core: 0.9.0.2 Illumina.BaseSpace.NativeApp.Host: 0.9.0.1 NDesk.Options: 0.2.1.0 Plugin assembly version information: Common.Logging: 2.1.2.0 CsvHelper: 2.0.0.0 GenerateFASTQ: 1.1.0.64 Illumina.BaseSpace.NativeApp.Core: 0.9.0.2 Illumina.BaseSpace.NativeApp.Isis: 0.9.31.1 Illumina.BaseSpace.NativeApp.Standard: 0.9.21.1 Illumina.BaseSpace.SDK.Private: 2.0.0.45 Illumina.BaseSpace.SDK: 2.0.0.189 Illumina.TerminalVelocity: 1.0.0.141 Mono.Posix: 4.0.0.0 Newtonsoft.Json: 6.0.0.0 ServiceStack.Common: 3.9.71.0 ServiceStack.Interfaces: 1.0.0.0 ServiceStack.Text: 3.9.71.0 [APP_LOG][2022-11-05 22:27:14.651]: Input Directory: /data/input [APP_LOG][2022-11-05 22:27:14.652]: Output Directory: /data/output [APP_LOG][2022-11-05 22:27:14.652]: Scratch Directory: /data/scratch [APP_LOG][2022-11-05 22:27:14.652]: Log Directory: /data/logs [APP_LOG][2022-11-05 22:27:19.584]: Launching Isis (attempt 1 out of 3) [APP_LOG][2022-11-05 22:27:19.584]: Running command: bash -c ""/opt/illumina/Isas/latest/Isas" -r "/data/scratch/workspace/RunFolder" -a "/data/scratch/workspace/RunFolder/Analysis"" Could not find mono directory as absolute or relative path. Checked these paths: Isas 1.0.10 -r /data/scratch/workspace/RunFolder -a /data/scratch/workspace/RunFolder/Analysis 2022-11-05T22:27:20,Workflow version 1.37.0: Starting Logging for /data/scratch/workspace/RunFolder/Analysis 2022-11-05T22:27:20,Run by user: root 2022-11-05T22:27:20,System 4412267d47cc with 36 cores and 59.0 GB of RAM 2022-11-05T22:27:20,ERROR: Workflow version 1.37.0: Starting Error Logging for /data/scratch/workspace/RunFolder/Analysis 2022-11-05T22:27:20,ERROR: Run by user: root 2022-11-05T22:27:21,Free gigabytes of disk space at /data/scratch/workspace/RunFolder/Analysis: 245.63 2022-11-05T22:27:21,Workflow: GenerateFASTQWorker Version=1.37.0 2022-11-05T22:27:24,Getting set of tile numbers from TileSet in RunInfo.xml. 2022-11-05T22:27:24,Running checkpoint 01: Generate FASTQ 2022-11-05T22:27:24,Running checkpoint 01.01: Generate FASTQ.Fastq Generation 2022-11-05T22:27:24,Launch process: /opt/illumina/Isas/1.37.0/bcl2fastq2/bin/bcl2fastq --ignore-missing-bcls --ignore-missing-filter --ignore-missing-positions --ignore-missing-controls --auto-set-to-zero-barcode-mismatches --find-adapters-with-sliding-window --adapter-stringency 0.9 --mask-short-adapter-reads 35 --minimum-trimmed-read-length 35 -R "/data/scratch/workspace/RunFolder" --sample-sheet "/data/scratch/workspace/RunFolder/SampleSheet.csv" -o "/data/scratch/workspace/RunFolder/Analysis/Temp_01.01-GenerateFASTQ.FastqGeneration" 2022-11-05T22:57:48,Max job memory (GB): 22.0 2022-11-05T22:57:48,Longest job runtime (Hours): 0.51 2022-11-05T22:57:48,>>>bcl2fastq2 call complete! 2022-11-05T22:57:48,>>>Total fastq generation time: 00:30:23.2 2022-11-05T22:57:48,Launch process: /opt/illumina/Isas/1.37.0/bcl2fastq2/bin/bcl2fastq --version 2022-11-05T22:57:49,Max job memory (GB): 0.9 2022-11-05T22:57:49,Longest job runtime (Hours): 0.00 2022-11-05T22:57:49,Elapsed time (step/time(sec)/name) 01.01 00:30:24.3 Generate FASTQ.Fastq Generation 2022-11-05T22:57:49,Saving checkpoint results to /data/scratch/workspace/RunFolder/Analysis/Checkpoints/01.01-GenerateFASTQ.FastqGeneration.json 2022-11-05T22:57:49,Elapsed time (step/time(sec)/name) 01 00:30:24.4 Generate FASTQ 2022-11-05T22:57:49,Saving checkpoint results to /data/scratch/workspace/RunFolder/Analysis/Checkpoints/01-GenerateFASTQ.json 2022-11-05T22:57:49,Saving Completed Job Information to /data/scratch/workspace/RunFolder/Analysis 2022-11-05T22:57:49,Total execution time: 00:30:24.4 2022-11-05T22:57:50,Free gigabytes of disk space at /data/scratch/workspace/RunFolder/Analysis: 277.82 2022-11-05T22:57:50,Total execution time: 00:30:28.7 [APP_LOG][2022-11-05 22:57:50.713]: Isis exited with exit code 0